切割率% 酶 AccI 寡核苷酸序列 2hr 20hr GGTCGACC CGGTCGACCG CCGGTCGACCGG 0 0 0 0 >90 >90 >90 >90 >90 50 >90 >90 10 >90 >90 0 75 25 0 0 50 0 0 25 25 0 0 >90 50 >90 >90 >90 >90 >90 0 0 0 0 >90 >90 >90 >90 >90 >90 >90 >90 25 >90 >90 0 >90 >90 0 0 >90 10 0 50 >90 0 0 >90 50 >90 >90 >90 >90 >90 AflIII CACATGTG CCACATGTGG CCCACATGTGGG AscI GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA AvaI CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA BamHI CGGATCCG CGGGATCCCG CGCGGATCCGCG BglII CAGATCTG GAAGATCTTC GGAAGATCTTCC BssHII GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA BstEII BstXI GGGT(A/T)ACCC AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT ClaI CATCGATG GATCGATC CCATCGATGG CCCATCGATGGG EcoRI GGAATTCC CGGAATTCCG CCGGAATTCCGG HaeIII GGGGCCCC AGCGGCCGCT TTGCGGCCGCAA >90 >90 HindIII CAAGCTTG CCAAGCTTGG CCCAAGCTTGGG 0 0 10 0 >90 >90 0 25 0 50 0 0 0 0 75 75 0 10 10 0 0 75 0 >90 >90 0 50 0 75 0 0 0 0 >90 >90 0 25 50 KpnI GGGTACCC GGGGTACCCC CGGGGTACCCCG MluI GACGCGTC CGACGCGTCG NcoI CCCATGGG CATGCCATGGCATG NdeI CCATATGG CCCATATGGG CGCCATATGGCG GGGTTTCATATGAAACCC GGAATTCCATATGGAATTCC GGGAATTCCATATGGAATTCCC NheI GGCTAGCC CGGCTAGCCG CTAGCTAGCTAG 切割率% 酶 寡核苷酸序列 2hr NotI 20hr TTGCGGCCGCAA ATTTGCGGCCGCTTTA AAATATGCGGCCGCTATAAA ATAAGAATGCGGCCGCTAAACTAT AAGGAAAAAAGCGGCCGCAAAAGGAAAA 0 10 10 25 25 10 >90 0 0 0 0 0 0 75 0 10 10 90 >90 >90 >90 0 25 >90 0 25 50 >90 NsiI TGCATGCATGCA CCAATGCATTGGTTCTGCAGTT PacI TTAATTAA GTTAATTAAC CCTTAATTAAGG PmeI GTTTAAAC GGTTTAAACC GGGTTTAAACCC AGCTTTGTTTAAACGGCGCGCCGG PstI GCTGCAGC TGCACTGCAGTGCA AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT 0 10 >90 >90 0 0 10 0 10 0 50 0 10 10 0 10 >90 >90 0 0 25 10 10 0 >90 0 50 75 PvuI CCGATCGG ATCGATCGAT TCGCGATCGCGA SacI SacII CGAGCTCG GCCGCGGC TCCCCGCGGGGA SalI GTCGACGTCAAAAGGCCATAGCGGCCGC GCGTCGACGTCTTGGCCATAGCGGCCGCGG ACGCGTCGACGTCGGCCATAGCGGCCGCGGAA ScaI GAGTACTC AAAAGTACTTTT 10 75 0 0 10 >90 10 10 0 0 0 0 10 >90 >90 >90 0 >90 75 75 25 75 10 10 50 >90 >90 >90 50 50 0 25 50 >90 >90 >90 0 >90 >90 >90 SmaI CCCGGG CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA SpeI GACTAGTC GGACTAGTCC CGGACTAGTCCG CTAGACTAGTCTAG SphI GGCATGCC CATGCATGCATG ACATGCATGCATGT StuI AAGGCCTT GAAGGCCTTC AAAAGGCCTTTT XbaI CTCTAGAG GCTCTAGAGC TGCTCTAGAGCA CTAGTCTAGACTAG XhoI CCTCGAGG CCCTCGAGGG CCGCTCGAGCGG 0 10 10 0 25 50 >90 0 25 75 0 75 >90 >90 XmaI CCCCGGGG CCCCCGGGGG CCCCCCGGGGGG TCCCCCCGGGGGGA
