专利内容由知识产权出版社提供
专利名称：OCULAR GENE THERAPY USING
AVALANCHE-MEDIATED TRANSFECTION
发明人：CHALBERG, Thomas, W., Jr.,BLUMENKRANZ,
Mark,PALANKER, Daniel, V.,VANKOV,Alexander,HUIE, Philip, Jr,MARMOR, Michael,F.,CALOS, Michele, P.
申请号：EP06801803.5申请日：20060815公开号：EP1924698A2公开日：20080528
摘要：The present invention provides a method of treating an ocular disease in asubject. In a first step, a nucleic acid is introduced into cells or a tissue. The nucleic acid isintroduced by electron avalanche transfection. With this technique, a high electric fieldinduces a vapor bubble and plasma discharge between an electrode and the surroundingmedium. The formation of a vapor bubble generates mechanical stress. Plasma dischargethrough the ionized vapor in the bubble enables connectivity between the electrode andthe surrounding medium, so that mechanical stress and electric field are appliedsimultaneously, which results in permeabilization of the cells or tissue. This
permeabilization in turn allows the nucleic acid to enter the cell or tissue. Cells or tissuecontaining the nucleic acid are then transplanted into an ocular region of the subject.
申请人：THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
地址：1705 El Camino Real Palo Alto, CA 94306-1106 US
国籍：US
代理机构：Coyle, Philip Aidan
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